Channel nuclear pore complex subunits are required for transposon silencing in Drosophila

Munafò, Marzia and Lawless, Victoria R and Passera, Alessandro and MacMillan, Serena and Bornelöv, Susanne and Haussmann, Irmgard U. and Soller, Matthias and Hannon, Gregory J and Czech, Benjamin (2021) Channel nuclear pore complex subunits are required for transposon silencing in Drosophila. eLife, 10. e66321. ISSN 2050-084X

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Abstract

The nuclear pore complex (NPC) is the principal gateway between nucleus and cytoplasm that enables exchange of macromolecular cargo. Composed of multiple copies of ~30 different nucleoporins (Nups), the NPC acts as a selective portal, interacting with factors which individually license passage of specific cargo classes. Here we show that two Nups of the inner channel, Nup54 and Nup58, are essential for transposon silencing via the PIWI-interacting RNA (piRNA) pathway in the Drosophila ovary. In ovarian follicle cells, loss of Nup54 and Nup58 results in compromised piRNA biogenesis exclusively from the flamenco locus, whereas knockdowns of other NPC subunits have widespread consequences. This provides evidence that some Nups can acquire specialised roles in tissue-specific contexts. Our findings consolidate the idea that the NPC has functions beyond simply constituting a barrier to nuclear/cytoplasmic exchange as genomic loci subjected to strong selective pressure can exploit NPC subunits to facilitate their expression.

Item Type: Article
Additional Information: ** From eLife via Jisc Publications Router ** History: collection 2021; received 07-01-2021; accepted 14-04-2021; pub-electronic 15-04-2021. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/
Identification Number: https://doi.org/10.7554/elife.66321
Date: 15 April 2021
Uncontrolled Keywords: Research Article, Cell Biology, Chromosomes and Gene Expression, transposon silencing, rna export, nuclear pore complex, nucleoporins, piwi-interacting RNAs, piRNAs, D. melanogaster
Subjects: C100 Biology
Divisions: Faculty of Health, Education and Life Sciences > School of Health Sciences
SWORD Depositor: JISC PubRouter
Depositing User: JISC PubRouter
Date Deposited: 24 May 2021 08:23
Last Modified: 24 May 2021 08:23
URI: http://www.open-access.bcu.ac.uk/id/eprint/11655

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