Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in different stage colorectal tumors
Söylemez, Zafer and Arikan, Evrim Suna and Solak, Mustafa and Arikan, Yüksel and Tokyol, Çiğdem and Şeker, Hüseyin (2021) Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in different stage colorectal tumors. Turkish Journal Of Medical Sciences, 51 (2). pp. 661-674. ISSN 1303-6165
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Abstract
Background/aim: The aim of the study is to assess expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in tumors and peripheral bloods of colorectal cancer patients in stages I–IV.
Materials and methods: The mRNA levels of the genes were determined in tumor tissues and peripheral blood samples of 45 colorectal cancer patients and colon tissues and peripheral blood samples of 5 healthy individuals. Real-time polymerase chain reaction method was used for the analysis.
Results: The mRNA level of the CPEB4 gene was significantly downregulated in colorectal tumor tissues and was upregulated in the peripheral blood of colorectal cancer patients relative to the controls (P < 0.05). APC mRNA level was significantly downregulated in tissues and upregulated in the peripheral blood (P < 0.05). TRIP13 mRNA level was upregulated in peripheral blood and also significantly upregulated in colorectal tumor tissues (P < 0.05). EIF2S3 mRNA level was upregulated in tissues and also significantly upregulated in peripheral blood (P < 0.05). PIK3CA mRNA level was downregulated in tissues and upregulated in peripheral blood. EIF4A1 mRNA level was downregulated in tissues and significantly upregulated in peripheral blood (P < 0.05). CTNNB1 mRNA level was downregulated in tissues and upregulated in peripheral blood. IFNg mRNA level was upregulated in both colorectal cancer tumor tissues and peripheral blood.
Conclusion: TRIP13 and CPEB4 mRNA up regulation in the peripheral blood of patients with colorectal cancer may be a potential target for early stage diagnosis. In addition to this evaluation, although there is not much study on EIF2S3 and EIF4A1 mRNA changes in cases with colorectal cancer, upregulation in peripheral blood draws attention in our study. These data will shed light on the new comprehensive studies.
| Item Type: | Article | ||||||||
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| Identification Number: | https://doi.org/10.3906/sag-2010-18 | ||||||||
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| Uncontrolled Keywords: | Biomarker, colorectal tumor, different stage, gene expression | ||||||||
| Subjects: | CAH01 - medicine and dentistry > CAH01-01 - medicine and dentistry > CAH01-01-01 - medical sciences (non-specific) CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-07 - genetics CAH09 - mathematical sciences > CAH09-01 - mathematical sciences > CAH09-01-03 - statistics |
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| Divisions: | Faculty of Computing, Engineering and the Built Environment > School of Computing and Digital Technology | ||||||||
| Depositing User: | Huseyin Seker | ||||||||
| Date Deposited: | 24 Jun 2021 09:27 | ||||||||
| Last Modified: | 12 Jan 2022 12:51 | ||||||||
| URI: | https://www.open-access.bcu.ac.uk/id/eprint/11814 |
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