1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen
Subramani, Indra Gandi and Perumal, Veeradasan and Gopinath, Subash C. B. and Mohamed, Norani Muti and Ovinis, Mark and Sze, Lim Li (2021) 1,1'-Carbonyldiimidazole-copper nanoflower enhanced collapsible laser scribed graphene engraved microgap capacitive aptasensor for the detection of milk allergen. Scientific Reports, 11. ISSN 2045-2322
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Abstract
The bovine milk allergenic protein, ‘β-lactoglobulin’ is one of the leading causes of milk allergic reaction. In this research, a novel label-free non-faradaic capacitive aptasensor was designed to detect β-lactoglobulin using a Laser Scribed Graphene (LSG) electrode. The graphene was directly engraved into a microgapped (~ 95 µm) capacitor-electrode pattern on a flexible polyimide (PI) film via a simple one-step CO2 laser irradiation. The novel hybrid nanoflower (NF) was synthesized using 1,1′-carbonyldiimidazole (CDI) as the organic molecule and copper (Cu) as the inorganic molecule via one-pot biomineralization by tuning the reaction time and concentration. NF was fixed on the pre-modified PI film at the triangular junction of the LSG microgap specifically for bio-capturing β-lactoglobulin. The fine-tuned CDI-Cu NF revealed the flower-like structures was viewed through field emission scanning electron microscopy. Fourier-transform infrared spectroscopy showed the interactions with PI film, CDI-Cu NF, oligoaptamer and β-lactoglobulin. The non-faradaic sensing of milk allergen β-lactoglobulin corresponds to a higher loading of oligoaptamer on 3D-structured CDI-Cu NF, with a linear range detection from 1 ag/ml to 100 fg/ml and attomolar (1 ag/ml) detection limit (S/N = 3:1). This novel CDI-Cu NF/LSG microgap aptasensor has a great potential for the detection of milk allergen with high-specificity and sensitivity.
| Item Type: | Article | ||||||
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| Identification Number: | https://doi.org/10.1038/s41598-021-00057-4 | ||||||
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| Uncontrolled Keywords: | materials science, nanoscience and technology | ||||||
| Subjects: | CAH10 - engineering and technology > CAH10-03 - materials and technology > CAH10-03-05 - biotechnology | ||||||
| Divisions: | Faculty of Computing, Engineering and the Built Environment > College of Engineering | ||||||
| Depositing User: | Mark Ovinis | ||||||
| Date Deposited: | 27 Sep 2022 15:34 | ||||||
| Last Modified: | 20 Jun 2024 11:50 | ||||||
| URI: | https://www.open-access.bcu.ac.uk/id/eprint/13613 |
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