Association between Interleukin-6 Gene Polymorphism (rs1800795 and rs1800796) and Type 2 Diabetes Mellitus in a Ghanaian Population: A Case-Control Study in the Ho Municipality

Obirikorang, Christian and Lokpo, Sylvester Yao and Owiredu, William K. B. A. and Ahenkorah-Fondjo, Linda and Osei-Yeboah, James and Duedu, Kwabena and Adejumo, Esther Ngozi and Ametepe, Samuel and Asamoah, Evans Adu and Coffie, Shadrack Asiedu and Mawuli, Emmanuel Nattah and Essandoh, Priscilla and Kwadzokpui, Precious Kwablah and Aihara, Ken-ichi (2024) Association between Interleukin-6 Gene Polymorphism (rs1800795 and rs1800796) and Type 2 Diabetes Mellitus in a Ghanaian Population: A Case-Control Study in the Ho Municipality. BioMed Research International, 2024. ISSN 2314-6133

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Background. There is no conclusive evidence on the association between interleukin- (IL-) 6 gene polymorphism and type 2 diabetes mellitus (type 2 DM). Thus, this study is aimed at evaluating the role of rs1800795 and rs1800796 polymorphisms in the pathogenesis of type 2 DM among Ghanaians in the Ho Municipality. Materials and Methods. We recruited into this hospital-based case-control study 174 patients with type 2 DM (75 DM alone and 99 with DM+HTN) and 149 healthy individuals between 2018 and 2020. Demographic, lifestyle, clinical, anthropometric, and haemodynamic variables were obtained. Fasting blood samples were collected for haematological, biochemical, and molecular analyses. Genomic DNA was extracted, amplified using Tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) technique, and genotyped for IL-6 gene polymorphism. Logistic regression analyses were performed to assess the association between IL-6 gene polymorphism and type 2 DM. Results. The minor allele frequency (MAF) of the rs1800795 and rs1800796 polymorphisms was higher in DM alone (57.5%, 62.0%) and DMwith HTN groups (58.3%, 65.3%) than controls (33.1%, 20.0%). Carriers of the rs1800795GC genotype (aOR = 2 35, 95% CI: 1.13-4.90, p =0022) and mutant C allele (aOR =241, 95% CI: 1.16-5.00, p=0019) as well as those who carried the rs1800796GC (aOR =867, 95% CI: 4.00-18.90, p<0001) and mutant C allele (aOR =884, 95% CI: 4.06-19.26, p=0001) had increased odds of type 2 DM. For both polymorphisms, carriers of the GC genotype had comparable levels of insulin, HOMA-IR, and fasting blood glucose (FBG) with those who carried the GG genotype. IL-6 levels were higher among carriers of the rs1800796GC variant compared to carriers of the rs1800796GG variant (p =0023). The rs1800796 polymorphism, dietary sugar intake, and exercise status, respectively, explained approximately 3% (p =0046), 3.2% (p =0038, coefficient = 1456), and 6.2% (p =0004, coefficient = −2754) of the variability in IL-6 levels, suggesting weak effect sizes. Conclusion. The GC genotype and mutant C allele are risk genetic variants associated with type 2 DM in the Ghanaian population. The rs1800796 GC variant, dietary sugar intake, and exercise status appear to contribute significantly to the variations in circulating IL-6 levels but with weak effect sizes.

Item Type: Article
Identification Number:
5 April 2024Accepted
26 April 2024Published Online
Subjects: CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-01 - biosciences (non-specific)
Divisions: Faculty of Health, Education and Life Sciences > School of Health Sciences
Depositing User: Gemma Tonks
Date Deposited: 09 May 2024 15:24
Last Modified: 09 May 2024 15:24

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