Chromosome position effects on gene expression in Escherichia coli K-12

Bryant, Jack A and Sellars, Laura E and Busby, Stephen J W and Lee, David J. (2014) Chromosome position effects on gene expression in Escherichia coli K-12. Nucleic acids research, 42 (18). pp. 11383-92. ISSN 1362-4962

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Abstract

In eukaryotes, the location of a gene on the chromosome is known to affect its expression, but such position effects are poorly understood in bacteria. Here, using Escherichia coli K-12, we demonstrate that expression of a reporter gene cassette, comprised of the model E. coli lac promoter driving expression of gfp, varies by ∼300-fold depending on its precise position on the chromosome. At some positions, expression was more than 3-fold higher than at the natural lac promoter locus, whereas at several other locations, the reporter cassette was completely silenced: effectively overriding local lac promoter control. These effects were not due to differences in gene copy number, caused by partially replicated genomes. Rather, the differences in gene expression occur predominantly at the level of transcription and are mediated by several different features that are involved in chromosome organization. Taken together, our findings identify a tier of gene regulation above local promoter control and highlight the importance of chromosome position effects on gene expression profiles in bacteria.

Item Type:	Article
Identification Number:	10.1093/nar/gku828
Dates:	Date Event 10 September 2014 Published 30 August 2014 Accepted 5 June 2014 Submitted
Subjects:	CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-02 - biology (non-specific) CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-07 - genetics CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-04 - microbiology and cell science
Divisions:	Faculty of Health, Education and Life Sciences > College of Health and Care Professions
Depositing User:	David Lee
Date Deposited:	14 Jan 2020 08:51
Last Modified:	12 Jan 2022 11:34
URI:	https://www.open-access.bcu.ac.uk/id/eprint/8712

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