A quadruplet 3-D laser scribed graphene/MoS2, functionalised N2-doped graphene quantum dots and lignin-based Ag-nanoparticles for biosensing

Mugashini, Vasudevan and Veeradasan, Perumal and Pandian, Bothi Raja and Mohamad Nasir, Mohamad Ibrahim and Hooi-Ling, Lee and Subash, CB Gopinath and Ovinis, Mark and Karuppanan, Saravanan and Phaik Ching, Ang and Natarajan, Arumugam and Raju, Suresh Kumar (2023) A quadruplet 3-D laser scribed graphene/MoS2, functionalised N2-doped graphene quantum dots and lignin-based Ag-nanoparticles for biosensing. International Journal of Biological Macromolecules, 253. ISSN 0141-8130

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Abstract

Troponin I is a protein released into the human blood circulation and a commonly used biomarker due to its sensitivity and specificity in diagnosing myocardial injury. When heart injury occurs, elevated troponin Troponin I levels are released into the bloodstream. The biomarker is a strong and reliable indicator of myocardial injury in a person, with immediate treatment required. For electrochemical sensing of Troponin I, a quadruplet 3D laserscribed graphene/molybdenum disulphide functionalised N2-doped graphene quantum dots hybrid with ligninbased Ag-nanoparticles (3D LSG/MoS2/N-GQDs/L-Ag NPs) was fabricated using a hydrothermal process as an enhanced quadruplet substrate. Hybrid MoS2 nanoflower (H3 NF) and nanosphere (H3 NS) were formed independently by varying MoS2 precursors and were grown on 3D LSG uniformly without severe stacking and restacking issues, and characterized by morphological, physical, and structural analyses with the N-GQDs and AgNPs evenly distributed on 3D LSG/MoS2 surface by covalent bonding. The selective capture of and specific interaction with Troponin I by the biotinylated aptamer probe on the bio-electrode, resulted in an increment in the charge transfer resistance. The limit of detection, based on impedance spectroscopy, is 100 aM for both H3NF and H3 NS hybrids, with the H3 NF hybrid biosensor having better analytical performance in terms of linearity, selectivity, repeatability, and stability.

Item Type:	Article
Identification Number:	10.1016/j.ijbiomac.2023.126620
Dates:	Date Event 28 August 2023 Accepted 7 September 2023 Published Online
Subjects:	CAH10 - engineering and technology > CAH10-01 - engineering > CAH10-01-06 - bioengineering, medical and biomedical engineering
Divisions:	Faculty of Computing, Engineering and the Built Environment > College of Engineering
Depositing User:	Mark Ovinis
Date Deposited:	11 Dec 2023 12:31
Last Modified:	07 Sep 2024 03:00
URI:	https://www.open-access.bcu.ac.uk/id/eprint/15036

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