Epithelium and stroma segmentation using multiscale superpixel clustering.

Landini, Gabriel and Fouad, Shereen and Galton, Antony and Randell, David (2018) Epithelium and stroma segmentation using multiscale superpixel clustering. In: 14th European Congress on Digital Pathology, 29th May - 1st June, 2018, Finland.

Full text not available from this repository. (Request a copy)

Abstract

Introduction: Accurate image segmentation is essential in quantitative histopathology although challenging due to tissue complexity, heterogeneity and the uncertainty of scene contents. Because structures detected at certain resolutions do not always coincide with the resolution at which other features are detected, we investigated a multiscale approach of image segmentation. Method: We developed an unsupervised framework using multiscale superpixels and k-means clustering. Images were partitioned into increasingly sized superpixels and clustered into 3 classes based on tissue stain uptake after colour deconvolution (aiming to identify background, epithelium and stroma) in H&E oropharyngeal cancer TMA sections. To overcome arbitrary label assignments during clustering, labelled regions were sequentially matched across consecutive scale image pairs using the additive inverse of the Dice index to generate a ‘cost matrix’ for all label combinations. A bipartite graph matching algorithm identified the combination that minimised the matrix overall cost. Results: From the matched ensemble, labelling probability images were generated to compute a ‘most likely’ segmentation. Fifty-six micro-array images compared with annotated standards achieved an average Dice index of 0.76 (median 0.79). The ‘most likely’ segmentations versus the standard, achieved a higher Dice index than those of the smallest and largest scale results (71% and 95% of instances respectively) and the average Dice over all scales (in 93% of instances). Conclusions: Our unsupervised segmentation, on average, performs better than single resolution superpixel clusterings. The method can also be applied to samples stained by other methods, once the dye RGB vectors (for colour deconvolution) have been appropriately determined.

Item Type:	Conference or Workshop Item (Poster)
Dates:	Date Event 29 May 2018 Published Online 29 March 2018 Accepted
Subjects:	CAH02 - subjects allied to medicine > CAH02-05 - medical sciences > CAH02-05-01 - medical technology CAH11 - computing > CAH11-01 - computing > CAH11-01-01 - computer science
Divisions:	Faculty of Computing, Engineering and the Built Environment Faculty of Computing, Engineering and the Built Environment > College of Computing
Depositing User:	Shereen Fouad
Date Deposited:	02 Apr 2019 09:05
Last Modified:	03 Mar 2022 15:46
URI:	https://www.open-access.bcu.ac.uk/id/eprint/7312

Actions (login required)

View Item