Facile synthesis of MoS2 nanoflower-Ag NPs grown on lignin-derived graphene for Troponin I aptasensing

Vasudevan, Mugashini and Remesh, Sathaniswarman and Perumal, Veeradasan and Raja, Pandian Bothi and Mohammad Ibrahim, Mohammad Nasir and Gopinath, Subash C. B. and Karuppanan, Saravanan and Ovinis, Mark (2023) Facile synthesis of MoS2 nanoflower-Ag NPs grown on lignin-derived graphene for Troponin I aptasensing. Chemical Engineering Journal, 468. p. 143613. ISSN 1385-8947

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This article presents the development and application of a green lignin-derived graphene biosensor for Troponin I, a biomarker for Acute Myocardial Infarction (AMI). The graphene was synthesized from oil palm lignin through an optimized laser scribing process. While the three-dimensional nature of the laser-scribed lignin-derived graphene (3D LSG) is advantageous, it suffers from poor electrical conductivity due to the amorphous nature of lignin. Therefore, semi-conductive molybdenum disulphide (MoS2) precursor with conductive green silver nanoparticles (Ag NPs) was added to 0.5, 1.0, 1.5, and 2.0 g of 3D LSG to synthesize 3D LSG_MoS2_Ag NPs hybrids via an aqueous hydrothermal process. Morphological, physical, and structural analyses showed the presence of petal-like MoS2 nanoflower with Ag NPs on the 3D LSG surface. The strong interrelation between 3D LSG, MoS2, and Ag NPs was confirmed by X-ray spectroscopy, Raman spectroscopy and energy dispersive spectroscopy (EDS). Specifically, X-ray spectroscopy revealed the formation of O1s, Ag 3d, C1s, Mo 3d, and S2p in the 3D LSG_MoS2_Ag NPs-2.0 hybrid. Raman spectroscopy revealed an enhancement in the surface area of the 3D LSG_MoS2_Ag NPs-2.0 hybrid, which enhances the detection sensitivity. The 3D LSG_MoS2_Ag NPs hybrid was subsequently chemically modified and immobilised with an aptamer to interact with Troponin I on an impedimetric sensor. The 3D LSG_MoS2_Ag NPs hybrid showed high analytical performance, high specificity, and a ∼ 4-fold increment in selectivity, with a detection limit of 100 attomolar. This biosensor has a sensitivity of 31.45 µA mM−1 cm−2, stability of 87%, with a relative standard deviation for reproducibility of 3.8%.

Item Type: Article
Identification Number: https://doi.org/10.1016/j.cej.2023.143613
16 May 2023Accepted
19 May 2023Published Online
Uncontrolled Keywords: Laser scribed graphene, Biopolymer, Nanosensor, Electrochemistry, Biomarker, 55 Acute Myocardial Infarction
Subjects: CAH10 - engineering and technology > CAH10-01 - engineering > CAH10-01-09 - chemical, process and energy engineering
Divisions: Faculty of Computing, Engineering and the Built Environment > College of Engineering
Depositing User: Mark Ovinis
Date Deposited: 26 May 2023 11:21
Last Modified: 20 Jun 2024 11:50
URI: https://www.open-access.bcu.ac.uk/id/eprint/14403

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