Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d

Knuckles, Philip and Lence, Tina and Haussmann, Irmgard U. and Jacob, Dominik and Kreim, Nastasja and Carl, Sarah H. and Masiello, Irene and Hares, Tina and Villaseñor, Rodrigo and Hess, Daniel and Andrade-Navarro, Miguel A. and Biggiogera, Marco and Helm, Mark and Soller, Matthias and Bühler, Marc and Roignant, Jean-Yves (2018) Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA-binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d. Genes & Development, 32 (5-6). pp. 415-429. ISSN 0890-9369

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Abstract

N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components of this complex, Flacc controls m6A levels and is involved in sex
determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA binding factor Nito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.

Item Type:	Article
Identification Number:	10.1101/gad.309146.117
Dates:	Date Event 13 March 2018 Published 12 February 2018 Accepted
Subjects:	CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-07 - genetics CAH03 - biological and sport sciences > CAH03-01 - biosciences > CAH03-01-08 - molecular biology, biophysics and biochemistry
Divisions:	Faculty of Health, Education and Life Sciences > College of Health and Care Professions
Depositing User:	Irmgard Haussmann
Date Deposited:	27 Nov 2019 08:04
Last Modified:	12 Jan 2022 11:37
URI:	https://www.open-access.bcu.ac.uk/id/eprint/7672

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