Mn3O4 Tetrahedral with Carbonyldiimidazole Nanoflower Deposition on Laser‐Scribed Graphene for Selective Bio‐Capture

Remesh, Sivainesh Devi and Chelvaraj, Jeysree and Perumal, Veeradasan and Gopinath, Subash C. B. and Ovinis, Mark and Karupannan, Saravanan and Arumugam, Natarajan and Kumar, Raju Suresh (2025) Mn3O4 Tetrahedral with Carbonyldiimidazole Nanoflower Deposition on Laser‐Scribed Graphene for Selective Bio‐Capture. Biotechnology and Applied Biochemistry. ISSN 0885-4513

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Abstract

Dengue fever, a mosquito‐borne viral infection, poses a significant global health challenge, particularly in tropical and subtropical regions. The absence of non‐effective vaccines and specific treatments underscores the need for advanced diagnostic tools for early detection and management. This study presents a novel biosensor for detecting dengue virus type 4 (DENV‐4) by combining carbonyldiimidazole nanoflower (CDI‐NF) with Mn 3 O 4 on laser‐scribed graphene (LSG). Material characterization techniques, including Raman spectroscopy, TEM, XRD, XPS, and FTIR, were employed to confirm the successful integration of Mn 3 O 4 and CDI‐NF, resulting in a unique 3D flower‐like structure. In order to verify the sensing efficiency, a selective DNA sample captured on LSG/Mn 3 O 4 ‐CDI‐NF was investigated for specifc binding with Aedes aegypti target DNA through selective hybridization and mismatch analysis. Electrochemical impedance studies further confirmed sensitive detection of up to 1 fM, where the sensitivity was confirmed by large transfer resistance ( R ct ) before and after hybridization with a regression coefficient 0.97373. EIS results demonstrated successful surface modifications and the biosensor's specificity in distinguishing between complementary, mismatched, and non‐complementary target sequences. The biosensor's ability to differentiate between these sequences highlights its potential for accurate and targeted DENV‐4 detection, offering a promising avenue for advancing dengue diagnostics.

Item Type:	Article
Identification Number:	10.1002/bab.2731
Dates:	Date Event 20 January 2025 Accepted 9 February 2025 Published Online
Uncontrolled Keywords:	dengue virus, electrochemical biosensor, laser-scribed graphene, surface functionalization
Subjects:	CAH10 - engineering and technology > CAH10-01 - engineering > CAH10-01-02 - mechanical engineering
Divisions:	Faculty of Computing, Engineering and the Built Environment > College of Engineering
Depositing User:	Gemma Tonks
Date Deposited:	11 Jul 2025 13:21
Last Modified:	11 Jul 2025 13:22
URI:	https://www.open-access.bcu.ac.uk/id/eprint/16512

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